The principal mission of the CCE is to develop and validate mathematical modeling strategies of tumor evolution and treatment response, utilize these strategies to identify best therapeutic intervention and make this expertise available to the DFCI community. We will investigate the treatment response of cancer cells and their microenvironment, develop novel mathematical frameworks describing the evolutionary dynamics of tumor progression and treatment response that are parameterized using the experimental systems, and predict and validate optimum intervention strategies that will ultimately be implemented as prospective clinical trials. This approach will incorporate many different parameters inherent to tumor cell populations to better define mechanisms of progression and resistance and develop alternative therapeutic strategies.